Cell Structure: Question 8
Syllabus 1.1
An electron micrograph of a pancreatic acinar cell includes a printed scale bar. The scale bar represents an actual distance of 1 μm and measures 4 mm in length when measured with a ruler on the printed image.
In the same image, a single mitochondrion measures 8 mm in length, and the width of a stack of Golgi membranes measures 0.6 mm.
(a) Use the scale bar to calculate the magnification of the micrograph. Show your working. [2]
(b) Calculate the actual length of the mitochondrion, giving your answer in micrometres (μm). [2]
(c) Calculate the actual width of the stack of Golgi membranes, giving your answer in nanometres (nm). [3]
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Worked solution
Part (a): Magnification from the scale bar
A scale bar’s printed length is an image size, and its labelled value is the corresponding actual size, so the same formula applies:
magnification = image size ÷ actual size
Both quantities must be in the same units. The actual size is given in micrometres, so convert the image size from millimetres to micrometres, since 1 mm = 1000 μm:
4 mm × 1000 = 4000 μm
Now divide by the actual size:
4000 μm ÷ 1 μm = 4000
So the magnification of the micrograph is ×4000.
Part (b): Actual length of the mitochondrion
Rearranging the formula to find actual size:
actual size = image size ÷ magnification
Convert the mitochondrion’s image length to micrometres:
8 mm × 1000 = 8000 μm
Divide by the magnification found in part (a):
8000 μm ÷ 4000 = 2 μm
So the actual length of the mitochondrion is 2 μm.
Part (c): Actual width of the Golgi stack, in nanometres
Convert the Golgi stack’s image width to micrometres:
0.6 mm × 1000 = 600 μm
Divide by the magnification:
600 μm ÷ 4000 = 0.15 μm
The question asks for the answer in nanometres, so convert micrometres to nanometres, since 1 μm = 1000 nm:
0.15 μm × 1000 = 150 nm
So the actual width of the Golgi stack is 150 nm.
Final answers
- (a) Magnification = ×4000.
- (b) Actual length of the mitochondrion = 2 μm.
- (c) Actual width of the Golgi stack = 150 nm.